Research Specialist

Baltimore, MD, United States

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The Coyne lab uses induced pluripotent stem cell (iPSC) derived neuron (iPSN) and postmortem human tissue models to understand the mechanistic basis and pathophysiologic consequences of impaired nuclear pore complex (NPC) and nuclear envelope surveillance and homeostasis in neurodegenerative diseases such as Amyotrophic Lateral Sclerosis (ALS) and Frontotemporal Dementia (FTD).

The Research Specialist will assist with various projects in the lab and primarily be responsible for the maintenance and differentiation of iPSNs, processing of samples for western blot and qRT-PCR and carrying out western blots and qRT-PCR experiments. The Specialist may also assist with immunostaining and confocal imaging as required. We are looking for a proficient Research Specialist with a sophisticated understanding of techniques and a solid foundation in the underlying science, gained from professional experience. The individual will work independently to carry out daily tasks with oversight from the PI.

The research technologist will participate in planning experiments and evaluating/interpreting test results utilizing detailed knowledge of research laboratory procedures and practices. The individual should be capable of independent judgment in the selection, development, performance and evaluation of research methods and operation of appropriate equipment.

Specific Duties & Responsibilities

Monitors biohazard safety standards and proper handling of potentially hazardous chemical and biological agents in the workplace.

Completes annual university biohazard/universal precaution safety training, as appropriate.

Uses universal safety precautions to protect self and co-workers from biohazardous materials, including blood-borne pathogens.

Plan and execute a defined set of experiments following discussion with the principal investigator.

Prepares solutions and reagents following standard laboratory formulas and procedures.

Will need to maintain excellent written records and electronic databases.

Performs general lab maintenance duties such as monitoring of inventory levels and proper storage of materials and supplies in accordance with established policies and procedures.

Participates in the organization and operation of the laboratory.

Uses various software applications, such as Excel spreadsheets, electronic mail, World Wide Web, statistical packages, and graphics packages to assemble and/or format data.

Follows established procedures or protocols and utilizes aseptic technique to perform laboratory experiments which include: iPSN culture (passaging and maintenance), qRT-PCR, western blotting, cloning and/or subcloning, immunostaining, and other basic cell and molecular biology techniques.

The Coyne lab uses induced pluripotent stem cell (iPSC) derived neuron (iPSN) and postmortem human tissue models to understand the mechanistic basis and pathophysiologic consequences of impaired nuclear pore complex (NPC) and nuclear envelope surveillance and homeostasis in neurodegenerative diseases such as Amyotrophic Lateral Sclerosis (ALS) and Frontotemporal Dementia (FTD).

The Research Specialist will assist with various projects in the lab and primarily be responsible for the maintenance and differentiation of iPSNs, processing of samples for western blot and qRT-PCR and carrying out western blots and qRT-PCR experiments. The Specialist may also assist with immunostaining and confocal imaging as required. We are looking for a proficient Research Specialist with a sophisticated understanding of techniques and a solid foundation in the underlying science, gained from professional experience. The individual will work independently to carry out daily tasks with oversight from the PI.

The research technologist will participate in planning experiments and evaluating/interpreting test results utilizing detailed knowledge of research laboratory procedures and practices. The individual should be capable of independent judgment in the selection, development, performance and evaluation of research methods and operation of appropriate equipment.

Specific Duties & Responsibilities

Monitors biohazard safety standards and proper handling of potentially hazardous chemical and biological agents in the workplace.

Completes annual university biohazard/universal precaution safety training, as appropriate.

Uses universal safety precautions to protect self and co-workers from biohazardous materials, including blood-borne pathogens.

Plan and execute a defined set of experiments following discussion with the principal investigator.

Prepares solutions and reagents following standard laboratory formulas and procedures.

Will need to maintain excellent written records and electronic databases.

Performs general lab maintenance duties such as monitoring of inventory levels and proper storage of materials and supplies in accordance with established policies and procedures.

Participates in the organization and operation of the laboratory.

Uses various software applications, such as Excel spreadsheets, electronic mail, World Wide Web, statistical packages, and graphics packages to assemble and/or format data.

Follows established procedures or protocols and utilizes aseptic technique to perform laboratory experiments which include: iPSN culture (passaging and maintenance), qRT-PCR, western blotting, cloning and/or subcloning, immunostaining, and other basic cell and molecular biology techniques.

Minimum Qualifications

Bachelor's Degree in Biology, Chemistry or related field.

One year laboratory experience performing relevant techniques.

Master's degree, with related graduate research, may substitute for experience to the extent permitted by the JHU equivalency formula.

Preferred Qualifications

Some knowledge of or experience with aseptic mammalian cell culturing preferable but not required.

Basic understanding of or experience with western blotting, qRT-PCR, or immunostaining preferable but not required.

Classified Title: Research Specialist

Role/Level/Range: ACRP/03/MA

Starting Salary Range: $16.92 - $29.61 HRLY ($38,000 targeted; Commensurate with experience)

Employee group: Full Time

Schedule: M-F/8:30am-5:00pm

Exempt Status:Non-Exempt

Location:School of Medicine Campus

Department name: Neurodegenerative and Injury Program

Personnel area: School of Medicine

Total Rewards

The referenced salary range is based on Johns Hopkins University’s good faith belief at the time of posting. Actual compensation may vary based on factors such as geographic location, work experience, market conditions, education/training and skill level. Johns Hopkins offers a total rewards package that supports our employees' health, life, career and retirement. More information can be found here: https://hr.jhu.edu/benefits-worklife/ .

Please refer to the job description above to see which forms of equivalency are permitted for this position. If permitted, equivalencies will follow these guidelines: JHU Equivalency Formula: 30 undergraduate degree credits (semester hours) or 18 graduate degree credits may substitute for one year of experience. Additional related experience may substitute for required education on the same basis. For jobs where equivalency is permitted, up to two years of non-related college course work may be applied towards the total minimum education/experience required for the respective job.

**Applicants who do not meet the posted requirements but are completing their final academic semester/quarter will be considered eligible for employment and may be asked to provide additional information confirming their academic completion date.

The successful candidate(s) for this position will be subject to a pre-employment background check. Johns Hopkins is committed to hiring individuals with a justice-involved background, consistent with applicable policies and current practice. A prior criminal history does not automatically preclude candidates from employment at Johns Hopkins University. In accordance with applicable law, the university will review, on an individual basis, the date of a candidate's conviction, the nature of the conviction and how the conviction relates to an essential job-related qualification or function.

The Johns Hopkins University values diversity, equity and inclusion and advances these through our key strategic framework, the JHU Roadmap on Diversity and Inclusion.

Equal Opportunity Employer

All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or status as a protected veteran.

Accommodation Information

If you are interested in applying for employment with The Johns Hopkins University and require special assistance or accommodation during any part of the pre-employment process, please contact the Talent Acquisition Office at [email protected] . For TTY users, call via Maryland Relay or dial 711. For more information about workplace accommodations or accessibility at Johns Hopkins University, please visit https://accessibility.jhu.edu/ .

The following additional provisions may apply, depending upon campus. Your recruiter will advise accordingly.

The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against Rubella (German measles), Rubeola (Measles), Mumps, Varicella (chickenpox), Hepatitis B and documentation of having received the Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.

School of Medicine - East Baltimore Campus

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