SCIENTIST/SENIOR SCIENTIST, PROTEIN SCIENCES

Redwood City, CA, United States

SCIENTIST/SENIOR SCIENTIST, PROTEIN SCIENCES

We are seeking an enthusiastic protein scientist with a strong passion for working at the bench, problem solving, and mentoring junior scientists. The candidate will work on a small team that is responsible for recombinant expression, purification, conjugation, and characterization of diverse proteins to support a wide range of projects in our pipeline. The ideal candidate will have expertise in protein engineering techniques, including phage, yeast, or mammalian cell display, experience optimizing expression and purification of antibody fragments (e.g. minibody and Fc-fusions) and have a working knowledge of antibody structure and function. They will work on diverse program teams and represent the protein sciences group as a subject matter expert. They should be highly organized, timeline-driven, and able to execute and drive projects to completion. This position is 100% on the bench.

KEY RESPONSIBILITIES:

Work alongside other team members in protein sciences to express, purify, and characterize diverse proteins ranging from antigens to engineered antibody-like molecules that meet project goals and timelines.

Oversee all activities related to recombinant protein expression in the group, including maintenance of Expi293 and ExpiCHO expression lines, and optimizing expression protocols to improve yield and/or solubility of proteins.

Develop and optimize high-throughput small-scale protein expression and purification methods to enable screening of antibodies and antibody fragments.

Optimize and troubleshoot expression and purification strategies for proteins that are challenging to produce.

Train and serve as a mentor at the bench for other scientists on the team.

Lead multiple efforts to design new expression and purification strategies for antibody fragments (e.g. minibody, scFv-IgG, scFv-Fc, diabody).

Serve as a subject matter expert in protein sciences in broader project-focused or group-focused team meetings.

Communicate regularly with cross-functional teams across Adicet on deliverables, timelines, and progress.

Lead efforts to improve notebook templates, write SOPs, and develop training documents.

Maintain and keep records of all data and studies according to existing templates.

Responsible for maintenance, training, and documentation for critical instrumentation in protein sciences.

QUALIFICATIONS:

PhD in chemistry, biochemistry, or chemical biology with at least 3-5 years of experience in an industry setting. MS with 10 years or more experience. Title will be dependent on experience.

Strong desire to work at the bench in a fast-paced and highly collaborative research environment.

Demonstrates a “can-do” attitude, can step up when necessary to assist other lab members without asking, willingness to help and improve lab environment for team members.

Must possess excellent verbal and written communication skills with prior experience serving as an SME on cross-functional teams.

Highly proficient in PowerPoint, word, outlook, and excel. Previous experience in Prism and/or Benchling a bonus, but not a requirement.

Candidate must be highly organized and detailed oriented and able to juggle competing projects and timelines.

Candidate must have extensive experience maintaining cell lines and have prior experience working in a BSC. Bonus if the candidate has previously used Expi293 and ExpiCHO cell lines for recombinant protein expression and/or has experience optimizing recombinant expression of proteins.

Significant prior experience maintaining and troubleshooting complex analytical equipment, such as HPLC, MALS, ViCell, AKTA Pure, Endosafe.

Deep understanding of protein engineering, antibody structure and its correlation to function.

Previous experience using display technologies (yeast, phage, or mammalian cell surface display) to isolate novel binders is a strong bonus.

Must have prior hands-on experience purifying antibodies and antibody fragments (e.g. minibody, diabody, IgG-scFv, scFv-Fc) using a variety of affinity resins (Protein A, Protein G, and Protein L).

Previous experience successfully developing methods to express and purify challenging proteins.

Possess a working knowledge and understanding of HIC, IEX, and SEC methods. Bonus if previous experience includes deep knowledge of different resins for each purification strategy and/or hands-on experience with all strategies.

Must be extremely comfortable developing methods, analyzing data, and optimizing purifications on AKTA systems (bonus if prior experience includes AKTA Pure).

Prior hands-on experience conjugating antibodies to fluorophores or other small molecules.

Bonus if prior experience includes conjugation using diverse chemistries (e.g. non-specific amine based labeling and site-specific labeling methods).

Prior experience with other functional assays (Kd determination by Octet, thermal stability measurements, long-term stability assays, protein ELISAs, western blots, dot blots, in-cell western assays, SEC-MALS)

BENEFITS:

Adicet Bio offers both comprehensive benefits and Paid Time (PTO) plans for employees, which begin on the first day of employment, as well as the opportunity to work in a growing, dynamic environment.

Adicet Bio is committed to creating a diverse environment and is proud to be an equal opportunity employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, gender, gender identity or expression, sexual orientation, national origin, genetics, disability, age, or veteran status.

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