Research Scientist - Genotoxicity

San Diego, CA, United States

Your Impact:

Poseida's proprietary gene-editing technologies, the Cas-CLOVER and Super piggyBac platforms, are the foundation for our next generation Gene Therapy products. You will work with an outstanding team of molecular biologists, protein engineers, gene-editing experts, pharmacologists, organic chemists, and formulation scientists to create new and effective therapeutic approaches. Your work will be central to our molecular discovery and pipeline program studies. You will have an opportunity to learn and advance in your career as this group is forward-thinking and fully supportive of development. We understand the meaning of team and are looking for an individual who wants to be a part of something bigger and a driving force in changing the lives of patients.

Position Summary:

Poseida is seeking an outstanding candidate to join our Gene Therapy team. The Genotoxicity Research Scientist will play a key role investigating the effects of gene editing and transposon integration using our platform technologies (Cas-CLOVER, piggyBac transposon, and site-specific piggyBac transposon) for therapeutic applications for gene therapies.

Responsibilities

These may include but are not limited to:

Apply your strong background in molecular biology, genetics, transposons and/or site-specific nucleases to characterize genetic manipulations generated using the piggyBac and Cas-CLOVER platforms.

Develop new and optimize existing customized NGS analysis tools for internal R&D use and in support of regulatory filings.

Generate extensive reports, for documentation of gene therapy/editing genotoxicity, suitable for regulatory filings.

Work in a highly collaborative, cross-functional team environment to perform experiments in the lab, analyze results, and communicate findings in team meetings.

Keep current on trends, new products and technologies in gene editing and knock-ins.

Requirements

Requirements, Knowledge, Skills, and Abilities: Ph.D. in a scientific discipline (e.g., molecular biology, cell biology, or related field) with 0-2 plus years of hands-on experience with gene editing, site-specific nucleases (especially RNA-guided nucleases), transposons, or other vectors used in vivo or in vitro, is required .

A strong background in standard molecular biology (such as PCR, qRT-PCR, ddPCR, DNA/RNA extraction and molecular cloning) is required.

Experience with mammalian cell culture is required, and any experience with the culture and genetic manipulation of primary human hepatocytes, organoids, T-cells, or other cells, is a plus.

Prior experience with viral or non-viral gene delivery technologies (transposon, episomal/plasmid, minicircle, or nanoparticles) is required.

Intimate knowledge or direct experience with sequencing library preparation (e.g. Illumina, Nanopore, or PacBio platforms) is strongly preferred.

Previous experience using methods to investigate/query off-target nuclease-mediated editing, such as GUIDE-seq, ONE-seq, Circle-seq, or amplicon-seq is preferred, but not required.

Previous experience using methods to investigate/query transposon or viral vector integration sites, such as LM-PCR or LAM-PCR, is preferred.

Proficiency with multi-parameter flow cytometry, cell sorting, and FACS analysis software is a plus.

Experience R, Python, shell scripting, and bioinformatics analysis, is a plus.

Must possess excellent oral and written communication skills.

Research Scientist (Genotoxicity) Pay Rate: $100,000 to $118,000 annually

The final pay offered to a successful candidate will be dependent on several factors that may include but are not limited to the type and length of experience within the job, type, and length of experience with the industry, education, geographic location, etc.

Who We Are and What We Do

Based in San Diego, Poseida Therapeutics is a clinical-stage biopharmaceutical company utilizing our unique and proprietary genetic engineering platform technologies to create next-generation cell and gene therapies with the capacity to cure. We are passionate about making an impact on patients' lives with the development of our CAR-T therapies in various cancers and gene therapies for rare diseases. Our goal is to deliver potential single treatment cures for patients in need. Our portfolio of product candidates is specifically designed to overcome the limitations of current generation cell and gene therapeutics.

At Poseida we put people first. Our team is passionate about improving patients' lives through innovation. See what our team has to say about our culture:

"My favorite part about Poseida is all the people that I get to work with. Everyone is so passionate about what we're doing here and there's so much camaraderie. It feels like one big enthusiastic family."

"My favorite part about working at Poseida is the amazing culture and working environment. Everybody is friendly, professional, collaborative, and has a remarkable can-do and positive attitude."

As we advance our compelling pipeline, there are endless opportunities for impact and growth. We encourage you to apply today!

Recruitment Fraud Alert

Alert: Beware of Employment Scams

Poseida Therapeutics, Inc. has received reports of employment-related scams. These scams have appeared in forms of false job advertisements and/or unsolicited contacts via communication/chat platforms, phone, email or text from individual(s) claiming to be or represent Poseida's Human Resources team. Be advised that Poseida Therapeutics does not extend unsolicited employment offers. Furthermore, Poseida Therapeutics does not request payment information or charge prospective employees with any fees during the recruitment process.

If you would like to pursue employment opportunities with Poseida Therapeutics, Inc., please visit our official careers website https://poseida.com/careers-culture/ or official Poseida Therapeutics LinkedIn page . Job postings that do not link directly to our careers website or official Poseida LinkedIn page are not legitimate and might be fraudulent. You may report fraudulent job advertisements or contacts via our Contact Us page at https://poseida.com/contact-us/ and select the subject "Careers." If you have been defrauded or suspect identity theft as a result of an employment scam, please contact your local law enforcement agency for guidance.

#J-18808-Ljbffr